Breakthrough in Aloe Vera Research: Micropropagation and Cytological Insights

Key Findings of the Study

  • Efficient Micropropagation Protocol: Researchers developed an effective method for Aloe vera shoot apices culture using Murashige and Skoog (MS) medium fortified with specific growth regulators.
  • Optimized Growth Conditions: The highest number of multiple shoot buds (4.36 ± 0.07) was observed in MS medium with 2.0 mg/l BAP + 1.0 mg/l IAA.
  • Successful Root Induction: The ideal rooting condition involved half-strength MS medium with 2.0 mg/l IBA, leading to a 95% acclimatization success rate in field conditions.
  • Chromosomal Stability in In Vitro Plants: Cytological studies confirmed that the karyotypic features of micropropagated plants closely resembled naturally grown plants, ensuring genetic stability.

Significance in Plant Biotechnology Micropropagation plays a crucial role in overcoming Aloe vera’s natural propagation challenges, such as slow growth and seed sterility. This research aligns with the recommendations of organizations like the International Society for Plant Pathology (ISPP), emphasizing the importance of plant tissue culture in enhancing genetic conservation and large-scale propagation.

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